Enrichment and Isolation of Surfactin-degrading Bacteria

A total of 100 environmental samples were investigated for their ability to degrade 1 g/L surfactin as a substrate. Among them, two enrichment cultures, which exhibited microbial growth well degradation, selected and further investigated. After several successive cultivations, nanopore sequencing full-length 16S rRNA genes with MinIONTM was used analyze the bacterial species in cultures. Variovorax spp., Caulobacter Sphingopyxis Pseudomonas spp. found be dominant these surfactin-degrading mixed Finally, one strain putida isolated bacterium. This degraded below detectable level within 14 days, C13 faster than C15 surfactin.